Mitochondrial DNA - (Methods in Molecular Biology) 3rd Edition by Matthew McKenzie (Paperback)
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<p/><br></br><p><b> Book Synopsis </b></p></br></br><p>Part I: <b> </b>Detection of Mitochondrial DNA Mutations and Mitochondrial DNA Damage<i></i></p><p>1. Comprehensive Mitochondrial Genome Analysis By Massively Parallel Sequencing</p><p>Meagan E. Palculict, Victor Wei Zhang, Lee-Jun Wong, and Jing Wang</p><p> </p><p>2. Analysis Of Translesion DNA Synthesis By The Mitochondrial DNA Polymerase g</p><p>William C. Copeland, Rajesh Kasiviswanathan, and Matthew J. Longley</p><p> </p><p>3. Quantification Of DNA Damage By Real-Time Qpcr</p><p>Wei Wang, Katja Scheffler, Ying Esbensen, <sup> </sup>And<sup> </sup>Lars Eide<sup></sup></p><p> </p><p>4. Quantitation Of Mitochondrial DNA Deletions Via Restriction Digestion/Long-Range Single Molecule</p> PCR<p></p><p>Yevgenya Kraytsberg, Xinhong Guo, Saisai Tao, Alexandra Kuznetsov, Catherine Mclean, Daniel Ehrlich, Evan Feldman, Igor Dombrovsky, Deye Yang, Gregory J. Cloutier, Carmen Castaneda-Sceppa, And Konstantin Khrapko</p><p> </p><p>Part II: Visualization of Mitochondrial DNA and Mitochondrial DNA Nucleoids</p><p>5. A Single-Cell Resolution Imaging Protocol Of Mitochondrial DNA Dynamics In Physiopathology, Mtrip, Which Also Evaluates Sublethal Cytotoxicity</p><p>Laurent Chatre, Benjamin Montagne and Miria Ricchetti</p><p> </p><p>6. Scalable Isolation Of Mammalian Mitochondria For Nucleic Acid And Nucleoid Analysis</p><p>Ken-Wing Lee and Daniel F. Bogenhagen</p><p>7. Tracking Mitochondrial DNA <i>In Situ</i></p><p>Anna Ligasová and Karel Koberna</p><p></p> <p></p><p>PART II: <i> </i>Analysis of Mitochondrial DNA Replication, Mitochondrial DNA-Encoded Protein Translation and Mitochondrial DNA Copy Number<i></i></p><p>8. Analysis Of Replicating Mitochondrial DNA By <i>In Organello</i> Labeling And Two-Dimensional Agarose Gel Electrophoresis </p><p>Ian J. Holt, Lawrence Kazak, Aurelio Reyes, and Stuart R. Wood</p><p> </p><p>9. Translation And Assembly Of Radiolabelled Mitochondrial DNA-Encoded Protein Subunits From Cultured Cells And Isolated Mitochondria</p><p>Luke E. Formosa, Annette Hofer, Christin Tischner, Tina Wenz, and Michael T. Ryan</p><p> </p><p>10. Analysis Of Mitochondrial DNA Copy Number And Its Regulation Through DNA Methylation Of <i>POLGA</i></p><p>Xin Sun, William Lee, Vijesh Vaghjiani, and Justin C. St. John<sup></sup></p><p> </p><p>Part VI: Modification Of Mitochondrial</p> DNA<p></p><p>11. Engineered Mtzfns For Manipulation Of Human Mitochondrial DNA Heteroplasmy</p>Payam A. Gammage, Lindsey Van Haute, and Michal Minczuk<p> </p><p>12. Generation Of Xenomitochondrial Embryonic Stem Cells For The Production Of Live Xenomitochondrial Mice</p><p>Ian A. Trounce, Jessica Ackerley, and Matthew McKenzie<sup></sup></p><p> </p><p>13. Import Of Fluorescent RNA Into Mitochondria Of Living Cells</p><p>Jaroslav Zelenka and Petr Jezek</p><p> </p><p>Part V: Purification of Proteins Involved In Mitochondrial DNA Replication and Transcription</p><p>14. Purification And Comparative Assay Of The Human Mitochondrial Replicative DNA Helicase</p>Fernando A. Rosado-Ruiz, Minyoung So, and Laurie S. Kaguni<p></p><p> </p><p>15. Expression And Purification Of Mitochondrial RNA Polymerase And Transcription Factor A From Drosophila Melanogaster</p><p>John P. Gajewski, Jamie J. Arnold, Tiina S. Salminen, Laurie S. Kaguni, and Craig E. Cameron</p><p> </p><p>16. Purification And Comparative Assay Of Human Mitochondrial Single-Stranded DNA-Binding Protein</p>Grzegorz L. Ciesielski, Fernando A. Rosado-Ruiz, and Laurie S. Kaguni<sup> </sup><p> </p><p>17. Biolayer Interferometry: A Novel Method To Elucidate Protein- Protein And Protein- DNA Interactions In The Mitochondrial DNA Replisome</p>Grzegorz L. Ciesielski, Vesa P. Hytönen, and Laurie S. Kaguni<p> </p><p> </p><p> </p><p/><br></br><p><b> From the Back Cover </b></p></br></br><p>This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. of <i>Mitochondrial DNA: Methods and Protocols </i>describes protocols for detecting mutations in mitochondrial DNA, techniques to assess mitochondrial DNA damage, visualization of mitochondrial DNA <i>in situ</i>, detection of mitochondrial DNA nucleoids within the mitochondria, methods for analyzing mitochondrial DNA replication, mitochondrial DNA-encoded protein translation and mitochondrial DNA copy number, the latest technologies for modifying the mitochondrial genome, and methods for the purification of proteins involved in the replication and transcription of mitochondrial DNA. Written for the <i>Methods in Molecular Biology</i> series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.</p><p> </p><p>Authoritative and practical, <i>Mitochondrial DNA: Methods and Protocols, Third Edition</i> will be useful not only for mitochondrial researchers but also for scientists studying human diseases where mitochondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration.</p>
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