Methods in Membrane Lipids - (Methods in Molecular Biology) 2nd Edition by Dylan M Owen (Paperback)
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<p/><br></br><p><b> Book Synopsis </b></p></br></br><p>1. Introduction to Membrane Lipids</p><p>Richard M. Epand</p><p> </p><p>2. Introduction: Membrane properties (Good) for Life</p><p>Marek Cebecauer</p><p> </p><p>3. Introduction To Fluorescence Probing Of Biological Membranes</p><p>Alexander P. Demchenko, Guy Duportail, Sule Oncul, Andrey S. Klymchenko, and Yves Mély<sup></sup></p><p> </p><p>4. The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs)</p><p>Charles Cranfield, Sonia Carne, Boris Martinac, <sup> </sup>and Bruce Cornell<sup></sup></p><p> </p><p>5. Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells</p><p>Deborah A. Brown</p><p> </p><p>6. Isolation of Giant Plasma Membrane Vesicles for Evaluation of Plasma Membrane Structure and Protein Partitioning</p><p>KR Levental<sup> </sup>and I Levental</p><p> </p><p>7. Asymmetric Giant Lipid Vesicle Fabrication</p><p>Peichi C. Hu and Noah Malmstadt</p><p> </p><p>8. Cholesterol Depletion using Methyl-b-cyclodextrin</p><p>Saleemulla Mahammad and Ingela Parmryd</p><p> </p><p>9. A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture Systems</p><p>G. Ekin Atilla-Gokcumen and Ulrike S. Eggert<sup> </sup></p><p> </p><p>10. Imaging Membrane Order using Environmentally Sensitive Fluorophores</p><p>G Ashdown and DM Owen<sup></sup></p><p> </p><p>11. 3D Super-resolution Imaging by Localisation Microscopy </p><p>Astrid Magenau and Katharina Gaus</p><p> </p><p>12. Electron Microscopy Methods for Studying Plasma Membranes</p><p>Alison J. Beckett and Ian A. Prior</p><p> </p><p>13. Measuring Cytoskeleton and Cellular Membrane Mechanical Properties by Atomic Force Microscopy</p><p>Charles Roduit, Giovanni Longo, Giovanni Dietler, and Sandor Kasas</p><p> </p><p>14. Fluorescence Linear Dichroism Imaging for Quantifying Membrane Order</p><p>Richard KP Benninger</p><p> </p><p>15. Scanning Fluorescence Correlation Spectroscopy on Biomembranes</p><p>Eduard Hermann, Jonas Ries, and Ana J. García-Sáez</p><p> </p><p>16. X-ray Diffraction of Lipid Model Membranes</p><p>Arwen I.I. Tyler, Robert V. Law, and John M. Seddon </p><p> </p><p>17. Solid State NMR of Lipid Model Membranes</p><p>Arwen I.I. Tyler, James A. Clarke, John M. Seddon, and Robert V. Law</p><p> </p><p>18. Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis and Applications</p><p>Michael Carnell, Alex Macmillan, and Renee Whan<sup></sup></p><p> </p><p>19. The Laurdan Spectral Phasor Method To Explore Membrane Micro-Heterogeneity And Lipid Domains In Live Cells</p><p>Ottavia Golfetto, Elizabeth Hinde, and Enrico Gratton</p><p> </p><p>20. Cholesterol Behavior in Asymmetric Lipid Bilayers: Insights from Molecular Dynamics Simulations</p><p>Semen O. Yesylevskyy and Alexander P. Demchenko<sup></sup></p><p> </p><p>21. Computer Simulations Of Phase Separation In Lipid Bilayers and Monolayers</p><p>Svetlana Baoukina and D. Peter Tieleman </p><p> </p><p/><br></br><p><b> From the Back Cover </b></p></br></br><p>This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and <i>in silico</i>. Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties, and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful <i>Methods in Molecular Biology </i>series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.</p><p> Authoritative and practical, <i>Methods in Membrane Lipids, Second Edition </i>seeks to aid scientist in further study into membrane lipids.</p>
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