Nitrogen Fixation - (Methods in Molecular Biology) by Markus W Ribbe (Hardcover)
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<p/><br></br><p><b> About the Book </b></p></br></br>This book offers an in-depth overview of the methods that have been applied to studying the nitrogenase at a molecular level, ranging from genetic, biochemical, spectroscopic and chemical methods to theoretical calculations.<p/><br></br><p><b> Book Synopsis </b></p></br></br><p><b>Part I: Nitrogen Fixation</b></p><p><b> </b></p><p><b>1. </b><b>Historic Overview of Nitrogenase Research</b></p><p> Yilin Hu and Markus W. Ribbe</p><p> </p><p><b>2. </b><b>Mechanism of Mo-Dependent Nitrogenase</b></p><p> Zhi-Yong Yang, Karamatullah Danyal, and Lance C. Seefeldt</p><p> </p><p><b>3. </b><b>Assembly of Nitrogenase MoFe Protein</b></p><p> Chi-Chung Lee, Aaron W. Fay, Jared A. Wiig, Markus W. Ribbe, and Yilin Hu</p><p> </p><p><b>4. </b><b>Genomic Analysis of Nitrogen Fixation</b></p><p> Ina P. O'Carroll and Patricia C. Dos Santos<b></p><p><b> </b></p><p><b>5. </b><b>Enzymatic Systems with Homology to Nitrogenase</b></p><p> Markus J. Bröcker and Jürgen Moser</p><p> </p><p><b>Part II: Genetic and Biochemical Methods</b></p><p><b> </b></p><p><b>6. </b><b>Molecular Biology and Genetic Engineering in Nitrogen Fixation</b></p><p> Patricia Dos Santos</p><p> </p><p><b>7. </b><b>Purification of Nitrogenase Proteins</b></p><p> Jared A. Wiig, Chi-Chung Lee, Aaron W. Fay, Yilin Hu, and Markus W. Ribbe</p><p> </p><p><b>8. Assays of Nitrogenase Reaction Products</b></p><p> William E. Newton and Michael J. Dilworth</p><p> </p><p><b>9. Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductase</b></p><p> Markus J. Bröcker and Jürgen Moser</p><p> </p><p><b>Part III: </b><b>Spectroscopic Methods</b><b></b></p><p><b> </b></p><p><b>10. X-Ray Crystallography</b></p><p> Lauren E. Roth and F. Akif Tezcan</p><p> </p><p><b>11. </b><b>X-Ray Absorption Spectroscopy</b></p><p> Serena DeBeer</p><p> </p><p><b>12. Small Angle X-Ray Scattering Spectroscopy</b></p><p> David W. Mulder and John W. Peters<b></p><p><b> </b></p><p><b>13. </b><b>Electron Paramagnetic Resonance Spectroscopy</b></p><p> Karamatullah Danyal, Zhi-Yong Yang, and Lance C. Seefeldt</p><p> </p><p><b>14. Magnetic Circular Dichroism Spectroscopy</b></p><p> Brian J. Hales<b></p><p><b> </b></p><p><b>15. Mössbauer Spectroscopy</b></p><p> Boi Hanh Huynh</p><p> </p><p><b>Part IV: Chemical M</b><b>ethods</b></p><p><b> </b></p><p><b>16. </b><b>Protocols for Cofactor Isoation of Nitrogenase</b></p><p> Aaron W. Fay, Chi-Chung Lee, Jared A. Wiig, Yilin Hu, and Markus W. Ribbe</p><p> </p><p><b>17. </b><b>Techniques for Functional and Structural Modeling of Nitrogenase</b></p><p> Patrick L. Holland<b></p><p><b> </b></p><p><b>Part V: Theoretical Calculations</b></p><p><b> </b></p><p><b>18. </b><b>Nitrogenase Structure and Function Relationships by Density Functional Theory</b></p><p> Travis V. Harris and Robert K. Szilagyi<b></p><p><b> </b></p><p><b>19. </b><b>Modeling the MoFe Nitrogenase System with Broken Symmetry Density Functional Theory</b></p><p> Gregory M. Sandala and Louis Noodleman</p><p/><br></br><p><b> From the Back Cover </b></p></br></br><p>Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable ammonia. In <i>Nitrogen Fixation: Methods and Protocols</i>, recognized experts in the field provide an up-to-date, in-depth overview of the methods that have been applied to studying the nitrogenase at a molecular level, ranging from genetic, biochemical, spectroscopic, and chemical methods to theoretical calculations. In addition, techniques used to study an enzyme system that is homologous to nitrogenase are described in this book. Written in the highly successful <i>Methods in Molecular Biology(TM)</i> series format, methods chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.</p><p> </p>Practical and cutting-edge, <i>Nitrogen Fixation: Methods and Protocols</i> will be useful for anyone interested in nitrogenase research and willing to venture further toward addressing the remaining mechanistic and biosynthetic questions of this fascinating enzyme system.
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