<p/><br></br><p><b> About the Book </b></p></br></br>This Methods in Molecular Biology(TM) book covers techniques for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, and protocols for analyzing data obtained using microsatellites.<p/><br></br><p><b> Book Synopsis </b></p></br></br><p>1. Microsatellites: Evolution and Contribution</p><p> Panagiotis Madesis, Ioannis Ganopoulos, and Athanasios Tsaftaris</p><p> </p><p>Part I: Discovery and Development of Microsatellites</p><p> </p><p>2. Screening of Genomic Libraries</p><p> Valdenice M. Novelli, Mariângela Cristofani-Yaly, Marinês Bastianel, Dario A. Palmieri, and Marcos A. Machado</p><p> </p><p>3. PCR-Based Identification of Microsatellite Arrays (PIMA)</p><p> Heng-Sheng Lin and Song-Bin Chang</p><p> </p><p>4. Fast Isolation by AFLP of Sequences Containing Repeats (FIASCO)</p><p> Kaisa Rikalainen</p><p> </p><p>5. Microsatellite DNA Capture from Enriched Libraries</p><p> Elena G. Gonzalez and Rafael Zardoya</p><p> </p><p>6. Next-Generation Sequencing for High-Throughput Molecular Ecology: A Step-by-Step Protocol for Targeted Multilocus Genotyping by Pyrosequencing</p><p> Jonathan B. Puritz and Robert J. Toonen</p><p> </p><p>7. Optimizing Selection of Microsatellite Loci from 454 Pyrosequencing Via Post-Sequencing Bioinformatic Analyses</p><p> Iria Fernandez-Silva and Robert J. Toonen</p><p> </p><p>8. Identification of DNA-Microsatellite Markers for the Characterization of Somatic Embryos in <i>Quercus suber</i></p><p> Arancha Gómez-Garay, Ma Ángeles Bueno, and Beatriz Pintos</p><p> </p><p>Part II: Amplification and Visualization</p><p> </p><p>9. Simple Sequence Repeats (SSR) Amplification</p><p> Kundapura V. Ravishankar and Bommisetty Padmakar</p><p> </p><p>10. Microsatellite Amplification in Plants: Optimization Procedure of Major PCR Components</p><p> Sana Ghaffari and Nejib Hasnaoui</p><p> </p><p>11. Development of a Multiplex PCR Assay for Characterization of Embryonic Stem Cells</p><p> Rajarshi Pal, Murali Krishna Mamidi, Anjan Kumar Das, Mahendra Rao, and Ramesh Bhonde</p><p> </p><p>12. Agarose Gel Electrophoresis and Polyacrylamide Gel Electrophoresis for Visualization of Single Sequence Repeats</p><p> James Anderson, Drew Wright, and Khalid Meksem</p><p> </p><p>Part III: Automated Capillary Sequencers</p><p> </p><p>13. Microsatellite Fragment Analysis Using the ABI Prism(R) 377 DNA Sequencer</p><p> Mark A. Renshaw, Melissa Giresi, and J. Adams</p><p> </p><p>14. Robust and Inexpensive SSR Markers Analyses Using LI-COR DNA Analyzer</p><p> Maria del Rosario Herrera and Marc Ghislain</p><p> </p><p>15. The Use of the MegaBACE for Sequencing and Genotype Analysis</p><p> Pamela A. Burger</p><p> </p><p>16. Analyzing Microsatellites Using the QIAxcel System</p><p> Deborah A. Dean, Phillip A. Wadl, Denita Hadziabdic, Xinwang Wang, and Robert N. Trigiano</p><p> </p><p>Part IV: Scoring and Data Analysis</p><p> </p><p>17. Microsatellite Analysis of Malaria Parasites</p><p> Pamela Orjuela-Sánchez, Michelle C.C. Brandi, and Marcelo U. Ferreira</p><p> </p><p>18. Informativeness of Microsatellite Markers</p><p> M. Humberto Reyes-Valdés</p><p> </p><p>19. Microsatellite Data Analysis for Population Genetics</p><p> Kyung Seok Kim and Thomas W. Sappington</p><p> </p><p>20. Molecular Mapping and Breeding with Microsatellite Markers</p><p> David A. Lightfoot and Muhammad J. Iqbal</p><p/><br></br><p><b> From the Back Cover </b></p></br></br><p>Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems. <i>Microsatellites: Methods and Protocols</i> brings together experts in the field to cover this significant area of research. Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful <i>Methods in Molecular Biology(TM)</i> series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.</p><p> </p>Authoritative and invaluable, <i>Microsatellites: Methods and Protocols</i> aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis.
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