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Product info

<p/><br></br><p><b> From the Back Cover </b></p></br></br>This collection explores detailed experimental protocols necessary for setting up a variety of <i>in vitro</i> cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the <i>Methods in Pharmacology and Toxicology</i> series, chapters include helpful background information on the <i>in vitro</i> assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading. <br>Authoritative and practical, <i>Cytochrome P450: In Vitro Methods and Protocols</i> serves as a key guide for researchers in the area of discovery and development of new medicines.

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