Arabidopsis Protocols - (Methods in Molecular Biology) 3rd Edition by Jose J Sanchez-Serrano & Julio Salinas (Hardcover)
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<p/><br></br><p><b> About the Book </b></p></br></br>This book features cutting-edge methods and protocols, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, and tips on troubleshooting and avoiding known pitfalls.<p/><br></br><p><b> Book Synopsis </b></p></br></br><p>Part I. Growing Arabidopsis</p><p> </p><p>1. Handling Arabidopsis Plants: Growth, Preservation of Seeds, Transformation and Genetic Crosses</p><p>Luz Rivero, Randy Scholl, Nicholas Holomuzki, Deborah Crist, Erich Grotewold, and Jelena Brkljacic</p><p> </p><p>2. Using Arabidopsis Related Model Species (ARMS): Growth, Genetic Transformation and Comparative Genomics</p><p>Giorgia Batelli, Dong-Ha Oh, Matilde Paino D'Urzo, Francesco Orsini, Maheshi Dassanayake, Jian-Kang Zhu, Hans J. Bohnert, Ray A. Bressan and Albino Maggio</p><p> </p><p>3. Growing Arabidopsis in vitro: Cell Suspensions, in vitro Culture, and Regeneration</p><p>Bronwyn J. Barkla, Rosario Vera-Estrella, and Omar Pantoja</p><p> </p><p> </p><p>Part II. Arabidopsis resources</p><p> </p><p>4. Arabidopsis Database and Stock Resources</p><p>Donghui Li, Kate Dreher, Emma Knee, Jelena Brkljacic, Erich Grotewold, Tanya Z. Berardini, Philippe Lamesch, Margarita Garcia-Hernandez, Leonore Reiser and Eva Huala</p><p> </p><p>5. Bioinformatic Tools in Arabidopsis Research</p><p>Miguel de Lucas, Nicholas J. Provart, and Siobhan Brady</p><p> </p><p>Part III. Genetic techniques</p><p> </p><p>6. Exploiting Natural Variation in Arabidopsis</p><p>Johanna A. Molenaar and Joost J. B. Keurentjes</p><p> </p><p>7. Grafting in Arabidopsis</p><p>Katherine Bainbridge, Tom Bennett, Peter Crisp, Ottoline Leyser, and Colin Turnbull</p><p> </p><p>8. Agrobacterium tumefaciens Mediated Transient Transformation of Arabidopsis thaliana Leaves</p><p>Silvina Mangano, Cintia Daniela González, and Silvana Petruccelli</p><p> </p><p>9. iTilling: Personalized Mutation Screen</p><p>Susan M. Bush and Patrick J. Krysan</p><p> </p><p>10. Tailor Made Mutations in Arabidopsis Using Zinc-Finger Nucleases</p><p>Yiping Qi, Colby G. Starker, Feng Zhang, Nicholas J. Baltes, and Daniel F. Voytas</p><p> </p><p>11. The Use of Artificial Micro RNA Technology to Control Gene Expression in Arabidopsis thaliana</p><p>Andrew L. Eamens, Marcus McHale, and Peter M. Waterhouse</p><p> </p><p>12. Generation and Identification of Arabidopsis EMS Mutants</p><p>Li-Jia Qu and Gengi Qin</p><p> </p><p>13. Generation and Identification of Arabidopsis T-DNA Insertion Mutants</p><p>Li-Jia Qu and Gengi Qin</p><p> </p><p>14. Identification of EMS-induced Causal Mutations in Arabidopsis thaliana by Next-Generation Sequencing</p><p>Naoyuki Uchida, Tomoaki Sakamoto, Masao Tasaka, and Tetsuya Kurata</p><p> </p><p>15. Arabidopsis Transformation with Large Bacterial Artificial Chromosomes </p><p>Jose M. Alonso and Anna N. Stepanova</p><p> </p><p>16. Global DNA Methylation Analysis Using Methyl-Sensitive Amplification Polymorphism (MSAP)</p><p>Mahmoud W. Yaish, Mingsheng Peng, and Steven J. Rothstein</p><p> </p><p>Part IV. Molecular biological techniques</p><p> </p><p>17. Next Generation Mapping of Genetic Mutations Using Bulk Population Sequencing</p><p>Ryan S. Austin, Steven P. Chatfield, Darrell Desveaux, and David S. Guttman</p><p> </p><p>18. Chemical Fingerprinting of Arabidopsis Using Fourier-transform Infrared (FT-IR) Spectroscopic Approaches </p><p>András Gorzsás and Björn Sundberg</p><p> </p><p>19. A Pipeline for 15N Metabolic Labeling and Phosphoproteome Analysis in Arabidopsis thaliana</p><p>Benjamin B. Minkoff, Heather L. Burch, and Michael R. Sussman</p><p> </p><p>20. Gene Expression Profiling Using DNA Microarrays</p><p>Kionoshin Maruyama, Kazuko Yamaguchi-Shinozaki, and Kazuo Shinozaki</p><p> </p><p>21. Forward Chemical Genetic Screening </p><p>Hyunmo Choi, Jun-Young Kim, Young Tae Chang, and Hong Gil Nam</p><p> </p><p>22. Highly-Reproducible ChIP-On-Chip Analysis to Identify Genome-Wide Protein Binding and Chromatin Status in Arabidopsis thaliana</p><p>Jong-Myong Kim, Taiko Kim To, Maho Tanaka, Takaho A. Endo, Akihiro Matsui, Junko Ishida, Fiona C. Robertson, Tetsuro Toyoda, and Motoaki Seki</p><p> </p><p> </p><p>Part V. Cell biological techniques</p><p> </p><p>23. Fluorescence Microscopy</p><p>Sebastien Peter, Klaus Harter<p/><br></br><p><b> From the Back Cover </b></p></br></br><p><i>Arabidopsis Protocols, Third Edition</i> compiles some of the most recent methodologies developed to exploit the Arabidopsis genome. These methodologies cover from the guided access to public resources, to genetic, cell biology, biochemical and physiological techniques, including both those that are widely used as well as those novel techniques likely to open up new avenues of knowledge in the future. In addition, considering the recent unparalleled progress of the "omics" tools in Arabidopsis, leading experts have contributed sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Arabidopsis thaliana is acknowledged as the most important plant model system by the scientific community and Arabidopsis research has fundamentally influenced our understanding of the basic biology and ecology of plants. Written in the successful <i>Methods in Molecular Biology</i> series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.</p><p> </p><p>Authoritative and easily accessible, <i>Arabidopsis Protocols, Third Edition</i> seeks to serve both experienced researchers and beginners with its detailed methodologies on this burgeoning scientific field. </p>
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