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<p/><br></br><p><b> Book Synopsis </b></p></br></br><p>1. <b>A Brief Review of West Nile Virus Biology<br></b><i>Berlin Londono-Renteria and Tonya M. Colpitts<br></i><i><br></i>2. <b>Overview of West Nile Virus Transmission and Epidemiology<br></b><i>Andrea Troupin and Tonya M. Colpitts<br></i><i><br></i>3. <b>Propagation and Titration of West Nile Virus on Vero Cells<br></b><i>Alexander J. McAuley and David W. C. Beasley<br></i><i><br>4. </i><b>Generating West Nile Virus from an Infectious Clone<br></b><i>Rianna Vandergasst and Brenda L. Frederickson<br></i><i><br></i>5. <b>Functional Analysis of West Nile Virus Proteins in Human Cells<br></b>Pakieli H. Kaufusi, Alanna Tseng, and Vivek R. Nerurkar<br><i><br></i>6. <b>Lessons from the Murine Models of West Nile Virus Infection<br></b><i>Brenna McGruder, Vandana Saxena, and Tian Wang<br></i><i><br></i>7. <b>Exploration of West Nile Virus Infection in Mouse Models<br></b><i>Penghua Wang<br></i><i><br></i>8. <b>Examination of West Nile Virus Neuroinvasion and Neuropathogenesis in the Central Nervous System of a Murine Model<br></b><i>Hameeda Sultana<br></i><i><br></i>9. <b><i>In Vitro</i> and <i>In Vivo</i> Blood-Brain Barrier Models to Study West Nile Virus Pathogenesis<br></b><i>Mukesh Kumar and Vivek R. Nerurkar<br></i><i><br>10. </i><b>Methodology for Identifying Host Factors Involved in West Nile Virus Infection<br></b><i>Manoj N. Krishnan<br></i><i><br></i>11. <b>ELISA and Neutralization Methods to Measure Anti-West Nile Virus Antibody Responses<br></b><i>Brian M. Friedrich and David W. C. Beasley<br></i><i><br></i>12. <b>Safe Handling of West Nile Virus in the Insectary</b><br><i>Dexter R. McKellar and Michael J. Conway<br></i><i><br>13. </i><b>Techniques for Experimental Infection of Mosquitoes with West Nile Virus<br></b><i>Yang Liu and Gong Cheng<br></i><i><br>14. </i>Field Surveillance Methods for West Nile Virus<br></b><i>Samuel B. Jameson, and Dawn M. Wesson<br></i><i><br><b>15. Detection Protocols for West Nile Virus in Mosquitoes, Birds, and Non-Human Mammals<br></b><i>Elizabeth B. Kauffman, Mary A. Franke, and Laura D. Kramer<br></i><i><br>16. </i><b>Field Methods and Sample Collection Techniques for the Surveillance of West Nile Virus in Avian Hosts<br></b><i>Sarah S. Wheeler, Walter M. Boyce, and William K. Reisen <br></i><i><br></i><b>17. Statistical Tools for the Interpretation of Enzootic West Nile Virus Transmission Dynamics<br></b><i>Kevin A. Caillouët and Suzanne Robertson<br></i><i><br></i><b>18. Role of Immune Aging in Susceptibility to West Nile Virus<br></b><i>Yi Yao and Ruth R. Montgomery</i><i><br></i><b>19. An Overview of Current Approaches Toward the Treatment and Prevention of West Nile Virus Infection</b><br><i>Dhiraj Acharya and Fengwei Bai</i></p><p></p><p/><br></br><p><b> From the Back Cover </b></p></br></br><p>This volume is a collection of research methods, techniques, and approaches to investigate the molecular biology of West Nile Virus (WNV). Chapters in the book cover many facets of WNV, such as propagation and titration of WNV in vero cells, examination of WNV neuroinvasion and neuropathogenesis in the central nervous system of a murine model, field surveillance methods for WNV, <b> </b>and <i>in vitro</i> and <i>in vivo</i> blood-brain barrier models to study WNV pathogenesis. A brief introduction, along with a discussion on WNV laboratory safety are also included. Written in the highly successful <i>Methods in Molecular Biology</i> series format, chapters includes introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. <p/>Practical and thorough, <i>West Nile Virus</i> will interest virologists, molecular biologists, microbiologists, and other scientists working with WNV. This book is a comprehensive guide for those conducting research in the field of WNV biology, including life cycles, pathogenesis, impact of infection, and possible treatment/prevention development.</p><p></p>

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