Quantitative Real-Time PCR - (Methods in Molecular Biology) by Roberto Biassoni & Alessandro Raso (Paperback)
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<p/><br></br><p><b> About the Book </b></p></br></br>Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ? Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation. --<p/><br></br><p><b> Book Synopsis </b></p></br></br><p>1. Twenty Years of qPCR: A Mature Technology?</p><p> Alessandro Raso and Roberto Biassoni</p><p> </p><p>2. Minimum Information Necessary for Quantitative Real-Time PCR Experiments</p><p> Gemma Johnson, Afif Abdel Nour, Tania Nolan, Jim Huggett, and Stephen Bustin</p><p> </p><p>3. Selection of Reliable Reference Genes for RT-qPCR Analysis</p><p> Jan Hellemans and Jo Vandesompele</p><p> </p><p>4. Introduction to Digital PCR</p><p> Francisco Bizouarn</p><p> </p><p>5. mRNA and micro RNA Purity and Integrity: The Key to Success in Expression Profiling</p><p> Benedikt Kirchner, Vijay Paul, Irmgard Riedmaier, and Michael W. Pfaffl</p><p> </p><p>6. Mediator Probe PCR: Detection of Real-Time PCR by Label Free Probes and a Universal Fluorogenic Reporter</p><p> Simon Wadle, Stefanie Rubenwolf, Michael Lehnert, Bernd Faltin, Manfred Weidmann, Frank Hufert, Roland Zengerle, and Felix von Stetten</p><p> </p><p>7. Absolute Quantification of Viral DNA: The Quest for Perfection</p><p> Domenico Russo and Mauro Severo Malnati</p><p> </p><p>8. A Multiplex Real-Time PCR-Platform Integrated into Automated Extraction Method for the Rapid Detection and Measurement of Oncogenic HPV Type-Specific Viral DNA Load from Cervical Samples</p><p> Francesco Broccolo</p><p> </p><p>9. Real-Time PCR Detection of <i>Mycoplasma pneumoniae </i>in the Diagnosis of Community-Acquired Pneumonia</p><p> Eddi Di Marco</p><p> </p><p>10. A Sensible Technique to Detect Mollicutes Impurities in Human Cells Cultured in GMP Condition</p><p> Elisabetta Ugolotti and Irene Vanni</p><p> </p><p>11. Real-Time Quantification Assay to Monitor <i>BCR-ABL1</i> Transcripts in Chronic Myeloid Leukemia</p><p>Pierre Foskett, Gareth Gerrard, and Letizia Foroni</p><p> </p><p>12. A Reliable Assay for Rapidly Defining Transplacental Metastasis Using Quantitative PCR</p><p> Samantha Mascelli</p><p> </p><p>13. Circulating Cell-Free DNA in Cancer</p><p> Pamela Pinzani, Francesca Salvianti, Claudio Orlando, and Mario Pazzagli</p><p> </p><p>14. Gene Expression Analysis by qPCR in Clinical Kidney Transplantation</p><p> Michael Eikmans, Jacqueline D.H. Anholts, and Frans H.J. Claas</p><p> </p><p>15. Post-Transcriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA and Micro RNA Data</p><p> Swanhild U. Meyer, Katharina Stoecker, Steffen Sass, Fabian J. Theis, and Michael W Pfaff</p><p> </p><p>16. Clinical Applications Using Digital PCR</p><p> Francisco Bizouarn</p><p> </p><p>17. Developing Non-Invasive Diagnosis for Single Gene Disorders: The Role of Digital PCR</p><p> Angela N. Barrett and Lyn S. Chitty</p><p/><br></br><p><b> From the Back Cover </b></p></br></br><p><i>Quantitative Real-Time PCR: Methods and Protocols</i> focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the <i>Methods in Molecular Biology</i> series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.</p><p> </p>Practical and authoritative, <i>Quantitative Real-Time PCR: Methods and Protocols</i> aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.<p/><br></br><p><b> Review Quotes </b></p></br></br><br><p>From the book reviews: </p>"The book is a valuable collection of qPCR-related details that cover a wide range of PCR uses. Aimed mainly at researchers (wet lab biologists) with a solid PCR background, the book provides enough details for the novice too, who may find the numerous examples as well as the end notes in each chapter very useful." (Irina Ioana Mohorianu, zbMATH, Vol. 1295, 2014)<br>
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