Exosomes and Microvesicles - (Methods in Molecular Biology) by Andrew F Hill (Paperback)
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<p/><br></br><p><b> Book Synopsis </b></p></br></br><p>Preface...<br> Table of Contents...<br> Contributing Authors...</p> <p>1. Methods to Analyze EVs<br>Bernd Giebel and Clemens Helmbrecht <p/>2. Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles<br>Sybren L. N. Maas, Marike L.D. Broekman, and Jeroen de Vrij <p/>3. Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis<br>Kym McNicholas and Michael Z. Michael <p/>4. Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy<br>Romain Linares, Sisareuth Tan, Céline Gounou, and Alain R. Brisson <p/>5. Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR<br>Shayne A. Bellingham, Mitch Shambrook, and Andrew F. Hill <p/>6. Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM(TM) System<br>Lesley Cheng and Andrew F. Hill <p/>7. A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration<br>Rong Xu, Richard J. Simpson, and David W. Greening <p/>8. Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array)<br>Rikke Bæk and Malene Møller Jørgensen <p/>9. Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic Activation<br>Karine Laulagnier, Charlotte Javalet, Fiona Hemming, and Rémy Sadoul <p/>10. A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space<br>R. Perez-Gonzalez, S.A. Gauthier, A. Kumar, M. Saito, M. Saito, and E. Levy <p/>11. Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission<br>Pascal Leblanc, Zaira E. Arellano-Anaya, Emilien Bernard, Laure Gallay, Monique Provansal, Sylvain Lehmann, Laurent Schaeffer, Graça Raposo and Didier Vilette <p/>12. Isolation of Platelet-Derived Extracellular Vesicles<br>Maria Aatonen, Sami Valkonen, Anita Böing, Yuana Yuana, Rienk Nieuwland and Pia Siljander <p/>13. Bioinformatics Tools for Extracellular Vesicles Research<br>Shivakumar Keerthikumar, Lahiru Gangoda, Yong Song Gho, and Suresh Mathivanan <p/>14. Preparation and Isolation of siRNA-Loaded Extracellular Vesicles<br>Pieter Vader, Imre Mäger, Yi Lee, Joel Z. Nordin, Samir EL Andaloussi, and Matthew J.A. Wood <p/>15. Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow Conditions<br>S.M. van Dommelen, M. Fish, A.D. Barendrecht, R.M. Schiffelers, O. Eniola-Adefeso, P. Vader</p><p/><br></br><p><b> From the Back Cover </b></p></br></br><p>This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful <i>Methods in Molecular Biology </i>series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. <p/> Practical and comprehensive, <i>Exosomes and Microvesicles: Methods and Protocols</i> is a valuable resource containing methodologies for anyone interested in researching EVs.</p><p/><br></br><p><b> About the Author </b></p></br></br>Andrew Hill, Associate Professor/Principle Research Fellow, Department of Biochemistry and Molecular Biology, The University of Melbourne Bio21 Institute, <br>Email: a.hill@unimelb.edu.au
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